Journal: Investigative ophthalmology & visual science

Article Title: Mesenchymal Stem Cells-Derived Exosomal miR-223-3p Alleviates Ocular Surface Damage and Inflammation by Downregulating Fbxw7 in Dry Eye Models.

doi: 10.1167/iovs.65.12.1

Figure Lengend Snippet: FIGURE 4. The miR-223-3p increased the number of goblet cells and inhibited conjunctival epithelial cells apoptosis. (A) Representative PAS staining of conjunctival goblet cells. Scale bar = 100 μm. (B) Goblet cell number. (C) Immunohistochemistry for MUC5AC in the conjunctiva. Scale bar = 50 μm. (D) MUC5AC-positive goblet cell density. (E) The mRNA levels of MUC5AC. (F) Representative images display TUNEL staining (in green) identifying apoptotic cells, along with DAPI staining (in blue) marking the nuclei in the conjunctiva. Scale bars = 50 μm. (G) Quantification of TUNEL-positive cells. Data were analyzed using ordinary 1-way ANOVA followed by Dunnett’s multiple comparisons test (B, D, and E) or Kruskal-Wallis test followed by Dunn’s multiple comparisons test (G). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.

Article Snippet: Sections were incubated in the anti-MUC5AC primary antibody (1:200, sc-21701; Santa Cruz, CA, USA) for 24 hours at 4°C, washed in PBS, and incubated with secondary antirabbit-HRP antibody.

Techniques: Staining, Immunohistochemistry, TUNEL Assay